Involvement of porin N,N-dicyclohexylcarbodiimide-reactive domain in hexokinase binding to the outer mitochondrial membrane.

Location of the dicyclohexylcarbodiimide-reactive glutamate residue in the bovine heart mitochondrial porin.

The mitochondrial porin or VDAC (Voltage-Dependent Anion Channel), the pore-forming structure responsible for the high permeability of the outer mitochondrial membrane, was found to be one of only three mitochondrial proteins bound by [14C]dicyclohexylcarbodiimide (DCCD) at low dosages (1.5 nmol/mg of mitochondrial porin) (De Pinto, V., Tommasino, M., Benz, R., and Palmieri, F. (1985) Biochim. ...

A COMPARATIW STUDY ON THE BINDING OF HEXOKINASE TO THE OUTER MITOCHONDRIAL MEMBRANE AND A HYDROPHOBIC MATRIX

Targeting of hexokinase 1 to liver and hepatoma mitochondria.

The proportion of hexokinase (HK; EC 2.7.1.1) isozyme 1 (HK1) that is bound to the outer mitochondrial membrane is tissue specific and developmentally regulated. HK activity is known to be markedly elevated in many cancer cells and a significant fraction is mitochondrial bound. This study examined the role of the 15-amino acid N-terminal domain of HK1 in binding to liver and hepatoma mitochondr...

Complexes between hexokinase, mitochondrial porin and adenylate translocator in brain: regulation of hexokinase, oxidative phosphorylation and permeability transition pore.

Introduction Hexokinase I binds to the mitochondrial surface of brain [l], muscle [2], fat [3], kidney [4] and liver cells [S]. Responsibility for this association lies with a specific binding protein [6], which has been identified as mitochondrial porin in the outer membrane [7,8] and is also known as voltage-dependent anion channel W A C ) [9]. The isolated outer membrane pore, when reconstit...

Involvement of Cytochrome P-450 in n-Butyl Nitrite-Induced Hepatocyte Cytotoxicity

      Addition of n-butyl nitrite to isolated rat hepatocytes caused an immediate glutathione depletion followed by an inhibition of mitochondrial respiration, inhi- bition of glycolysis and ATP depletion. At cytotoxic butyl nitrite concentrations, lipid  peroxidation  occurred  before  the  plasma  membrane  was  disrupted. Cytochrome P-450 inhibitors inhibited peroxynitrite formation and prev...